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Byregowda, S. M.
- Identification of Bovine Viral Diarrhoea Virus Type 2 in Cattle Bull Semen from Southern India and its Genetic Characterization
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Authors
Niranjan Mishra
1,
Semmannan Kalaiyarasu
1,
K. C. Mallinath
2,
Katherukamem Rajukumar
1,
Rohit K. Khetan
1,
Siddharth Gautam
1,
M. D. Venkatesha
2,
S. M. Byregowda
2
Affiliations
1 ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, IN
2 Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, IN
1 ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, IN
2 Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, IN
Source
Current Science, Vol 114, No 03 (2018), Pagination: 666-670Abstract
Although bovine viral diarrhoea virus (BVDV) is prevalent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n = 4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from southern India by real-time RT-PCR. Genetic typing of the 5'-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found genetically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of Npro sequences confirmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India highlights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission.Keywords
Bovine Viral Diarrhoea, Bull Semen, Genetic Characterization, Mandatory Testing.References
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- Survivin Expression in Canine Spontaneous Cutaneous and Subcutaneous Tumors and its Prognostic Importance
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Materials and Methods: Forty cases of canine cutaneous and subcutaneous tissue tumors on histopathological examination revealed various round cell, epithelial, and mesenchymal cell tumors. Survivin gene expression was detected in all tumors tested by TaqMan real-time polymerase chain reaction assay by comparative cycle threshold method.
Results: The mean survivin gene expression value of benign tumors was 0.94±0.63 folds and that of malignant tumors was 18.87±5.30 folds. Postsurgical follow up of 30 malignant tumor cases revealed death in 8, recurrence in 7, and neoplastic free alive status in 15 dogs with mean survivin fold difference values of 48.49±12.39, 14.63±6.37, and 5.034±2.27, respectively. The mean survivin gene expression value was significantly higher in malignant (30 cases, 18.87±5.30) compared to benign tumors (10 cases, 0.94±0.63), and it varied between various postsurgical follow-up groups (p<0.05). Survival analysis, using survivin gene expression median cutoff value of 3.74 in 30 malignant tumors, was performed to predict probable survival period in malignant cutaneous and subcutaneous tumors of dogs.
Conclusion: Results of the present study indicated that the expression of survivin in canine cutaneous and subcutaneous tumors has prognostic value, and survivin expression greater than median cutoff value of 3.74 has a poor prognosis.
Authors
N. Kavya
1,
S. Rao
1,
M. L. Sathyanarayana
1,
H. D. Narayanaswamy
1,
S. M. Byregowda
2,
L. Ranganath
3,
A. Kamaran
4,
K. M. Purushotham
2,
T. K. Kishore
1
Affiliations
1 Department of Veterinary Pathology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
2 Department of Biotechnology, Institute of Animal Health and Veterinary Biologicals, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
3 Department of Veterinary Surgery and Radiology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
4 Department of Veterinary Medicine, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
1 Department of Veterinary Pathology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
2 Department of Biotechnology, Institute of Animal Health and Veterinary Biologicals, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
3 Department of Veterinary Surgery and Radiology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
4 Department of Veterinary Medicine, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, IN
Source
Veterinary World, Vol 10, No 10 (2017), Pagination: 1286-1291Abstract
Aim: The present study was carried out to know the expression level of survivin, an inhibitor of apoptosis protein with an objective to determine its prognostic importance in cutaneous and subcutaneous tissue tumors of dogs.Materials and Methods: Forty cases of canine cutaneous and subcutaneous tissue tumors on histopathological examination revealed various round cell, epithelial, and mesenchymal cell tumors. Survivin gene expression was detected in all tumors tested by TaqMan real-time polymerase chain reaction assay by comparative cycle threshold method.
Results: The mean survivin gene expression value of benign tumors was 0.94±0.63 folds and that of malignant tumors was 18.87±5.30 folds. Postsurgical follow up of 30 malignant tumor cases revealed death in 8, recurrence in 7, and neoplastic free alive status in 15 dogs with mean survivin fold difference values of 48.49±12.39, 14.63±6.37, and 5.034±2.27, respectively. The mean survivin gene expression value was significantly higher in malignant (30 cases, 18.87±5.30) compared to benign tumors (10 cases, 0.94±0.63), and it varied between various postsurgical follow-up groups (p<0.05). Survival analysis, using survivin gene expression median cutoff value of 3.74 in 30 malignant tumors, was performed to predict probable survival period in malignant cutaneous and subcutaneous tumors of dogs.
Conclusion: Results of the present study indicated that the expression of survivin in canine cutaneous and subcutaneous tumors has prognostic value, and survivin expression greater than median cutoff value of 3.74 has a poor prognosis.